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Item #:
221926695
Phosphate Buffered Saline with Tween-20 (1), Lab PBST Buffer, 0.1um Filtration Sterilized, 7.4 pH, Ready to Use, 500ml/Bottle
Item #:
221926695
USD 26
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Reliable Formula: PBST offers pH buffering capacity, ideal for consistent lab results.
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What Stands Out
Ready To Use
Eliminates preparation time, offering immediate usability for laboratory applications, ensuring convenience for researchers and saving valuable time during experiments.
Sterile Filtration
Guaranteed 0.1um filtration sterilization that minimizes contamination risks, providing confidence and reliability in sensitive biological experiments.
Optimal pH Balance
Maintains a stable pH of 7.4, critical for biological stability, ensuring consistent conditions for various biochemical assays and processes.
Product Details
| Brand Name | CryoKing |
| Manufacturer | BIOLOGIX |
| Part Number | PBST-500-1 |
| Unit Count | 1.0 Count |
| Included Components | PBS Buffer with Tween-20 |
| Brand | CryoKing |
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Biological Researchers
This buffer is ideal for researchers conducting cellular and molecular biology experiments requiring a stable pH environment.
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Diagnostic Laboratories
Perfect for labs performing diagnostic assays that require a non-toxic, sterile environment for accurate results.
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Cell Culture Applications
Suitable for use in cell culture systems to maintain osmotic balance and support cell viability.
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High-Volume Productions
Not ideal for large-scale productions requiring bulk solutions, as this comes in a smaller 500ml bottle.
Product Description
Phosphate Buffered Saline with Tween-20 (1), Lab PBST Buffer, 0.1um Filtration Sterilized, 7.4 pH, Ready to Use, 500ml/Bottle
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Customer Questions & Answers
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Question:
What is Lab PBST Buffer used for?
Answer: Lab PBST Buffer is primarily used in biological and biochemical research. It's an essential washing buffer for immunoassays and cell culture, helping to maintain pH levels while reducing non-specific binding. The inclusion of Tween-20 aids in blocking and washing steps, making it especially useful for experiments such as ELISA and Western blotting, where protein interactions need to be meticulously controlled. -
Question:
What is the significance of the pH level in Lab PBST Buffer?
Answer: The pH level of 7.4 in the Lab PBST Buffer is crucial as it mimics physiological conditions, providing an optimal environment for biological reactions. Maintaining this pH helps prevent denaturation of proteins and ensures accurate experimental results. Ideal for working with cell cultures and sensitive assays, this pH level supports cellular functions and enzyme activities, making it suitable for a variety of applications in laboratories. -
Question:
How is the Lab PBST Buffer sterilized?
Answer: The Lab PBST Buffer is sterilized using a 0.1um filtration process, which effectively removes bacterial and fungal contaminants without affecting the buffer's chemical properties. This filtration method ensures that the buffer remains free from microbial life, making it safe for sterile procedures and sensitive experiments, especially in cell culture and immunohistochemistry applications. -
Question:
Can I dilute Lab PBST Buffer for my experiments?
Answer: Yes, Lab PBST Buffer is provided as a concentrated solution and can be diluted according to your specific experimental needs. Common practice involves diluting the buffer with sterile distilled water to achieve the desired concentration. This versatility allows researchers to tailor the buffer to their particular protocols, enhancing its effectiveness in various applications, such as washing steps in immunoassays. -
Question:
What are the benefits of using Tween-20 in the Lab PBST Buffer?
Answer: Tween-20 is a non-ionic detergent included in Lab PBST Buffer to reduce the surface tension and facilitate the washing process during experiments. It helps minimize non-specific binding of antibodies in immunoassays, improving the overall specificity and sensitivity of results. This makes it particularly effective in assays like ELISA and Western blotting, where precise interactions are critical. -
Question:
Is Lab PBST Buffer safe to use in cell culture?
Answer: Absolutely, Lab PBST Buffer is designed for use in cell culture applications. Its pH of 7.4 ensures compatibility with cellular environments, and the buffer's formulation allows for effective washing without harming the cells. Researchers often use it during co-culture experiments and during the preparation of samples for flow cytometry or microscopy, where cell viability is paramount. -
Question:
What are some typical applications of Lab PBST Buffer?
Answer: Lab PBST Buffer is commonly used in various applications such as Western blotting, ELISA, and immunofluorescence assays. Its formulation is tailored for washing steps, blocking agents, and other crucial processes in molecular biology. For example, in Western blots, it helps remove excess unbound antibodies, improving the clarity of protein detection and ensuring reliable results. -
Question:
What is the shelf life of Lab PBST Buffer?
Answer: The shelf life of Lab PBST Buffer, when stored properly, can be quite lengthy, typically lasting for several months. However, it's essential to check for any signs of contamination or changes in color before use. Researchers should always follow storage recommendations and use aseptic techniques to ensure the buffer maintains its integrity for experiments, especially in sensitive applications. -
Question:
Where can I buy Lab PBST Buffer, 500ml in Micronesia?
Answer: You can purchase Lab PBST Buffer, 500ml from Ubuy in Micronesia. Ubuy provides a convenient platform to find various lab supplies, including this specific buffer formulation. By shopping with Ubuy, you gain access to reliable products suited for your laboratory needs, ensuring you have the tools necessary for successful research and experimentation.
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Features & Benefits
- Reliable formula for consistent lab results.
- Enhanced performance with Tween-20 for effective washing.
- Low endotoxin levels achieved through one-step injection molding.
- Guaranteed batch consistency with rigorous testing.
- Each batch is validated for quality assurance.
- Long shelf life of at least 3 years when stored at room temperature.
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